Journal: Cells

Article Title: Development of a Custom Fluid Flow Chamber for Investigating the Effects of Shear Stress on Periodontal Ligament Cells

doi: 10.3390/cells13211751

Figure Lengend Snippet: Computational fluid flow shear stress simulation of the parallel flow chamber using Autodesk CFD (Autodesk, San Rafael, CA, USA). ( A ) The shear stress magnitude was determined to confirm the mathematical calculations and distribution of wall shear stress at 6 dyn/cm 2 across the desired chamber cell seeding area. Arrows represent the flow direction. ( B ) The velocity was simulated to determine undesirable phenomena such as turbulence. Streamline visualization of the flow field shows no flow turbulence at the seeding area of the flow chamber. Turbulence lines near the inlet and outlet are shown. ( C ) The region of consistent FSS was identified by fluid flow simulation (Autodesk CFD; Autodesk, San Rafael, CA, USA). The graphs depict FSS along the length (left) and across the middle of the chamber (right). A custom-made gasket was designed using Autodesk Inventor (Autodesk, San Rafael, CA, USA) (see for details).

Article Snippet: Using a 3D modeling CAD software (Autodesk Inventor Professional 2022; Autodesk, San Rafael, CA, USA) and computational fluid dynamic (CFD) software (Autodesk CFG 2023; Autodesk, San Rafael, CA, USA), the channel dimensions and the fluid flow parameters were optimized, resulting in a setup that applies wall shear stress of 6 dyn/cm 2 in a defined and reproducible manner.

Techniques: Shear